THE EFFECT OF ISOQUINOLINE ALKALOID ON SUPEROXIDE DISMUTASE ENZYME ACTIVITY OF RAT HEART MITOCHONDRIA

Abstract

Superoxide dismutase (SOD) is one of the main enzymes of the antioxidant system. SOD is a group of metalloenzymes that catalyze the dismutation reaction of superoxide anion radicals, keeping their concentration in the cell at a low level and reducing the probability of active singlet oxygen formation. Depending on the metal ion in the active center of the enzyme, several SOD isoenzymes are distinguished, among which Cu-, Zn-SOD has the greatest activity [1].
The harmful effects of the superoxide anion radical are also related to its participation in the formation of the highly reactive hydroxyl radical (Haber-Weiss reaction), which plays a leading role in the lipid peroxidation (LPO) process [2]. In order to maintain bioradicals at a physiological level, a complex multi-component antioxidant defense system including enzymes and low molecular compounds has been formed in the body [1]. An increase in the amount of SOD in heart mitochondria indicates an increase in the generation of free radicals. Bioactive compounds play an important role in increasing the activity of antioxidant enzymes.